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message posted on Thursday, June 9th, 2011

Conversation with a scientist...

I had a long conversation with Jean-Louis Jorissen Pharmacist of Comed, about his new product RONI. (Regular Oligosaccharides for Normalizing the Intestin)

It became a story about pre-, pro-, and antibiotics all playing a role in the well-known framework of his mission: The Resistance Selection Method, without antibiotics. Together with Willem De Bruijn, I had numerous long conversations about this approach. Willem was an early believer of the method. Himself being a dentist and his wife a doctor, both medical academic educated, where already long time ago, convinced the abuse of antibiotics could not continue like that.

A practical problem for Jean-Louis is that the products he manufactures are nutriceuticals, which cannot claim any medical effect. You cannot use the right words to tell the fanciers what the products can do says Jean-Louis. One cannot present it as medication. The use of the words blood, liver, Indication of prevention, are not allowed. All the claims have to be proved, where after the product should bee registered as medication and sold by pharmacists or vets.

The new RONI is a bio regulator of the digestive ecosystem. (Regular Oligosaccharides for Normalizing the Intestin)
This ecosystem is a sophisticated population settled in the intestine and its like with people: regretfully between the numerousness good ones, we have a limited couple of bad ones.


What’s the relation between the pigeon as a host and it’s flora?
The intestinal flora is composed of a huge number of (different) bacteria.
They take care of all the metabolic exchanges.
This intestinal flora as a whole is an extremely complex centre of the metabolism, able to influence strongly the condition of the host as well in the positive as in the negative way.
The so-called “microbiotum” (the total of the germ population) plays a protective roll against the colonizing by uninvited and disease provoking germs.
It is able to stimulate the immune system and to rule many different metabolisms.
The smallest changing in the balance between the saprophytes (living from trash, the good ones) and the pathogenic germs (the bad ones) can lead to sever problems
Stress by transport, age, hormonal modifications can disturb this balance.

What could be the consequences of the loss of balance?
Recent studies on rats showed that obese rats suffer of a loss of balance in their flora.
Opposite to the not obese rats, they have a flora poor in Bacteriodetes and rich in Firmicutes .
A low caloric diet, led to a significant increase of the bacetriodete flora.
In this experiment, the flora of obese rats was inoculated on ‘not obese’ rats.
After a couple of weeks, the ‘not obese’ rats took significant extra weight.
This shows there is an important link between intestinal flora and overweight.

Can the germs influence the intestinal flora?
The intestinal microbes’ biotope plays without any doubt an important role.
From this evident principal, diverse and multiple concepts where introduces in to the market:” balancing flora” or “stimulation of the beneficial flora”
It was always with the aim to change the bacterial population as well as the metabolism of the intestinal ecosystem.
Certain substances can influence the flora as follows:
1) by adding extra living germs from whom one supposes they will be useful and who thanks to their adaptation possibilities at the digestion conditions(very acid pH, salts and strong enzymes)  are able to reach different places in the digestive tube to become metabolic active
We call these the probiotics.
2) through indigestible substrates able to reach the colon certain bacteria, considered as good, can be fed to make them grow and digest.
We call these the prebiotics.
3) by  adding Allicin influences the fat digestion in the intestine and it is capable of penetrating bacterial cell walls and preventing the release of many enzymes that are toxic.

What are probiotics ?
The intestines contain a large amount of microorganisms called the micro-flora.
It maintains a symbiotic relationship with the host, protects it from infections, supports digestion, and produces useful metabolites. (e.g. for the immune system)
Hence, these are living microbial nutrients with a favourable influence regarding the host, by regulating the microbial balance.
In RONI Bacillus Subtilis was chosen, an approved strain according to the European Law.
The strain was prepared to retain its function in a hostile environment where bile-(salts) and strong digestive acids  are present.
Its presence is favourable for the population of Lactobacilli responsible for the production of lactic acid.
The latter suppresses the growth of Salmonella, Campylobacter, Clostridium , protozoa, the Coliforms and like.
What are prebiotics ?
Compared to the probiotics, importing bacteria from outside the system, the prebiotics have the advantage to modulate the own bacteria.
Therefore, they change the composition, of the natural ecosystem of these microorganisms that have a beneficial effect.
The concept of the prebiotcs is related to the development of the functional nutrition.
A prebiotc is a not digestible substance, triggering a favourable physiological effect for the host, by enabling specifically the growth or the activity of a limited number of populations already present in the intestine. 
Prebiotics are mixtures of natural hexoses( saccharides), present in fruits or vegetables.
These can also be made trough biosynthesis, starting from simple or multiple sugars.
These are extremely interesting because of their nutritional value.
We can ad easily 5 to 25 gr. per kg of food on daily bases.
These multiple sugars are resistant against hydrolyses (splitting) from the enzymes of he digestive system.
They are able to reach unchanged the colon where they serve as a substrate for the own flora.
The modifications on the flora, caused by this saccharides, have an influence on the functioning of the whole body.
Intake on regular basis of these compounds influences the intestinal flora the disturbing of the digestion, the immunity, the absorption of minerals and the concentration levels of plasma lipids.
The biochemical biodiversity in prebiotics finds its origin in the natural diversity and the numerous existing synthesis procedures.
Prebiotics are commonly oligosaccharides of small size only differing from each other trough the nature of the saccharides and their way of being bond among each other.
These parameters also define their physico-chemical .and physiological properties.
Their chemical structure of the oligosaccharides is off great importance for their use in bacterial strains, where it intervenes numerous functions e.g. the flora itself, the production of specific fatty acids and the intestinal transit .

Do prebiotics change the intestinal flora?
For a long time one was convinced, the bacterial composition of the intestinal flora was fixed definitively for every creature, in such a way that the nutrition could not modify this anymore.
The occurring of the prebiotics has shown this vision was not correct and that it was possible to influence the population of these bacteria at least for a certain period.
This influence has consequences on the metabolic activity of the intestinal flora, it conducts the fermentation and other enzymatic activities in numerous intestinal functions.
The fermentation in the colon by poly- and oligosaccharides leads to an increasing of the bio mass.
The best prove to detect the prebiotic effect of a substance, is by increasing the concentration in the droppings of dominant populations of lactic acid producing bacteria (like Bifidus bacteria in humans) 
The modifications in concentrations due to oligosaccharides in the intestinal flora have an influence on the whole of the metabolism.
Besides of the growth of bacteria trough digesting oligosaccharides, there are many different metabolites occurring with an extremely interesting effect on the complex intestinal balance.

Can those prebiotics contribute to the -for pigeons important fat digestion in the food?
They even do contribute at the so important production of fatty acids with short chain.
This happens mostly in the first part of the colon, where after they are transported to the last part.
These short chain fatty acids (SCFA) decrease the degree of acidity (pH) of the intestinal content.
This low pH decreases the growth of the pathogenic germs.
The SCFA derivates from propionates bock the growth of E.Coli and a number of Salmonella strains..
Their metabolites contribute to the conservation of the normal intestinal flora functions due to the digestion trough the wall cells of the colon, for which they are an important feed element.
95% of the SCFA are metabolized and absorbed in the colon.
Between the numerous roles attributed to the SCFA one has the improving of the blood circulation in the colon, a better intestinal motility (muscle) the transport of electrolytes and the decreasing of inflammation or infection.

Do prebiotics modify liquid droppings?
Their effect is due to the increasing of the bacterial mass and the inclusion of free water or bond to bacteria.
This increasing of continence of water modifies the consistence and the plasticity in the droppings.
This amount of extra water stored in the intestine is of great value during the race.

Are there other favourable effects besides a better absorption of fat or lipids?
The intestine is the crucial platform for numerous exchanges with the general circulation.
The prebiotics have the property of organising the absorption and conservation of different minerals e.g. calcium, magnesium but also iron.
In animal tests, studies have proven that the use of prebiotics in combination with calcium led to better bone structure and strong skeleton.
It is mainly due to a decreased pH caused by the fermentation of prebiotics.
This leads to a better solubility of minerals in the intestinal lumen and an improved absorption through the intestinal mucus surface.(wall)
One of the most important functions of the intestinal flora is the early fermentation of the indigestible substrates in the higher intestinal parts of the digestive system. 
The use of bio-regulatory oligosaccharides in rats, during four weeks showed there where different fermentations all depending of the inoculated bacteria.
These oligosaccharides are preferably fermented by Bifidobacteries and  bacteroïds and a lot less by Clostridium.
When these three strains are presented simultaneously, these oligosaccharides are completely fermented.
But, these oligosaccharides are more easily digested by Bifidus and bacteroïds then by Clostridium.
Clostridium being without any doubt the most unsafe and threatening strain for the intestinal flora, of all named germs..
Hence, we offer the flora a very favourable nutrition.
In tests on dogs, an increasing of the population of bacteroïds, was found after administration of RONI-oligosaccharides more than after administration of other oligosaccharides.
This proves that RONI contains a perfect substrate for bacteroïd populations, and its absorbtion making them grow in contrast with the other prebiotics improving the growth of Bifidobacteries.
This is an advantage for the host, because bacteroïds have a complete arsenal at their disposal of enzymes able to metabolize complex carbon hydrates making the digestion more easy an preventing a lot of problems.
On the intestinal digestive surface, they lay down a carpet, preventing the pathogenic germs themselves to get fixed at the host .

What is allicin and what is its effect?
Allicin extract from garlic trough the enzymatic effect due to allinase, is turned in to allicin.
Allicin and  derivates are effective against a wide spectrum of bacterial species, viral infections, fungal and protozoal disease as well as a large number of parasite problems.

The fermentation of certain oligosaccharides by bacteria of the intestinal flora can generate a number of metabolites who are very useful for the host.
Studies showed these oligosaccharides generate short chain fatty acids that decrease the pH in the droppings.
At the same time, the amount of water in the droppings increases, enabling a better transit and generating a buffer of free disposable water during the races.
By formation of SCFA (short chain fatty acids) and its decreasing of the pH, it prevents the settling of a pathogenic flora, often the cause of bad functioning of the digestion.
These SCFA’ s are of great importance for the absorption, not only of the minerals, but the SCFA themselves are better absorbed trough the intestinal mucus.
SCFA are the most important energy source liberating great amount of the indispensable ATP (Adenosin Triphosphate) supporting the muscle effort during the racing
The SCFA derivates from propionates are contributing in the battle against the colonizing of the flora by Salmonelle, E.Coli and many other unwished germs.


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Michel Vanlint
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